BioBrick Categories of Parts (BB Categories) Provides a list of part categories for inputinto the BB Library | |
BioBrick Prefix and Suffix Adder (Pre~Suf) Contains a cluster of Grasshopper components | |
BioBricks Library (BB Library) library of all the parts in the BioBricks registry. | |
BioBricks Prefix Standard (BB Pre) simply the sequence of the standard BioBricks prefix used to enable ligation with compatible sticky ends | |
BioBricks Prefix ATG (BB Pre ATG) simply the sequence of the standard BioBricks prefix used, with sequences starting with ATG, to enable ligation with compatible sticky ends | |
BioBricks Suffix (BB Suf) simply the sequence of the standard BioBricks suffix used to enable ligation with compatible sticky ends | |
E0240 GFP with RBS, and Terminator (GFP RET) RBS: B0032 + GFP Reporter: E0040 + Terminators: B0010 & B0012 | |
GFP with Promoter, RBS, and Terminator (GFP PRET) Tet Promotor R0040 + RBS: B0034 + GFP Reporter: E0040 + Terminators: B0010 & B0012 | |
Plasmid Backbones DNA (Backbones) Provides the DNA for teh 4 commonly use BioBrick backbones. | |
Plasmid Insert (Insert) Insert a gene into a plasmid backbone, optionall add in the BB prefix and suffix. Index reamins at beginning of gene; the prefix is at the end of the sequence. | |
Promoter DNA (Promoters) Provides a list of the 10 most commomnly used BioBrick promoters, and outputs the DNA of the selected part. | |
Protein Coding DNA (Coding) Provides a list of the 10 most commomnly used BioBrick protein codeing sequences, and outputs the DNA of the selected part. | |
Resistance to Chloramphenicol (CamR) DNA sequence to produce Chloramphenicol resistance | |
Resistance to Kanamycine (KanR) DNA sequence to produce Kanamycine resistance | |
Resistance to Tetracycline (TetR) DNA sequence to produce Tetracycline resistance. On BioBrick plasmid backbone pSB1T3 this gene is on the primary strand. | |
Resitance to Amp (AmpR) DNA sequence to produce Ampicillin resistance | |
RFP with RBS, and Terminator (RFP RET) RBS: B0034 + RFP Reporter: E1010 + Terminators: B0010 & B0012 | |
Ribosome Binding Site DNA (RBS) Provides a list of the 10 most commomnly used BioBrick RBS, and outputs the DNA of the selected part. | |
Teminator DNA (Terminators) Provides a list of the 10 most commomnly used BioBrick terminators, and outputs the DNA of the selected part. | |
YFP with RBS, and Terminator (YFP RET) RBS: B0034 + YFP Reporter: E0030 + Terminators: B0010 & B0012 |
Complementary Strand (Comp) creates a complementary strand based in the input sequence | |
DNA Clean gets rid of anything that is not a, A,T,G, or C and capitalizes all characters | |
DNA Find (Find) Finds a sequence within a sequence and return the domain of its location. | |
Primer Design (Primer Des) to find a primer with good melt temp and GC content and avoiding bad hairpins | |
Random DNA (Ran DNA) create a ramndom string of GTAC | |
Reverse DNA (Rev) reverses the sequence of DNA | |
Reverse Subdomain (Rev Sub) reverses the position and direction of a subdomain |
E. coli Optimization (E.coli Opt) create an optimized RNA sequence for E coli to produce the input AA chain. | |
Simple Transcription (Tx) simple conversion DNA to RNA changing T to U | |
Simple Translation (Tl) converts RNA into Amino Acids | |
Transcription Promoter to Terminator (Transcription) Transcribes DNA to RNA starting just after the promoter site and ending just prior to the termitator site | |
Transcription σ Converts DNA to RNA based on -35 and -10 sites | |
Translation Translates RNA to amino acid chain with options for finding the proper reading frame |
Amino Acid Visualizer (AA Viz) Creates and displays an offset complementary strand | |
DNA Subdomain Visualizer (DNA Sub Viz) Visualizes the specified subdomain of a strand | |
DNA Complementary Strand Visualizer (DNA Comp Viz) Creates and displays an offset complementary strand | |
DNA Complementary Subdomain Visualizer (DNA Comp Sub Viz) Visualizes the specified subdomain of a created offset complementary strand | |
DNA Visualizer (DNA Viz) Visualizes the DNA in the Rhino viewport |
PDB Sort (PDB Sort) sort protein data base file | |
PDB Open make the pdb info accessible | |
PDB Plot create a visual energy plot of a pdb file | |
Protein Query Search the RCSB Protein DataBase for matching Amino Acis sequences and the address of a corresponding .pdb file. | |
Protein Ribbon visualizes the structure of a protein |
A Simple Ligation (Lig) Concatenates two DNA sequences | |
Ligate Ligate tests to see if 2 strands have matching sticky ends. Is sothey are joined together. If both ends connect, plasmid is set to true. | |
Sticky Ends (Sticky) identifies the overhanging ends of DNA. |
Enzyme List (Enzymes) A list of enzymes to choose from that passes the proper index for the Restriction Enzymes output object | |
Restriction Enzyme Digest (Digest) Cuts up DNA based on target sits of the input restriction enzyme and outputs double stranded DNA with sticky ends. | |
Restriction Enzymes Cut Locations (Restrict. Enzymes) outputs the cut locations of any major restriction enzyme. use the Enzymes List to choose the enzyme you want or enter the index if you know it already |
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