We need your help! 🔥
Please consider disabling your ad-blocker to support this website! 🔑
We are a free, community-supported website! 🥰
We only display small unobtrusive ads which help us stay online! 💯
Thank you in advance! ❤️
Digest
Library
Ligate
Protein
Transcription/Translation
Utilities
Visualize

Primer Design

to find a primer with good melt temp and GC content and avoiding bad hairpins

Inputs

NameIDDescriptionType
TextDNAContains a collection of text fragmentsString
CurveCrvContains a collection of generic curvesCurve
IntegerStContains a collection of integer numbersInteger
IntegerLContains a collection of integer numbersInteger
IntegerShContains a collection of integer numbersInteger

Outputs

NameIDDescriptionType
PrimerPResulting textString
TempTtarget melt temp is 55-65, this reading is onoy accurate around 20-40 nucleotidesGoo
GC%GC%The result of the MultiplicationGoo
Hair Pin CountsHPhair pin counts at different lengthsGoo

Site design © Robin Rodricks.   Alba and associated data © 2024 Ryan Hoover.  
Rhinoceros and Grasshopper are registered trademarks of Robert McNeel & Associates.  Hosted by GitHub

Report an Issue  |  Terms of Service